10.06.2005, 17:59
Nutritional Immunology
Dietary Fructooligosaccharides Increase Intestinal Permeability in Rats
Sandra J. M. Ten Bruggencate, Ingeborg M. J. Bovee-Oudenhoven1, Mischa L. G. Lettink-Wissink and Roelof Van der Meer
Nutrition and Health Program, Wageningen Center for Food Sciences/NIZO Food Research, 6710 BA Ede, The Netherlands
1To whom correspondence should be addressed. E-mail: Ingeborg.Bovee@NIZO.nl .
We showed previously that fructooligosaccharides (FOS) decrease the resistance to salmonella infection in rats. However, the mechanism responsible for this effect is unclear. Therefore, we examined whether dietary FOS affects intestinal permeability before and after infection with Salmonella enterica serovar Enteritidis. Male Wistar rats were fed restricted quantities of a purified diet that mimicked the composition of a Western human diet. The diet was supplemented with 60 g/kg cellulose (control) or 60 g/kg FOS and with 4 mmol/kg of the intestinal permeability marker chromium EDTA (CrEDTA) (n = 8 or 10). After an adaptation period of 2 wk, rats were orally infected with 108 colony-forming units (cfu) of S. enteritidis. Mucin concentrations in intestinal contents and mucosa were measured fluorimetrically, as markers of mucosal irritation. Intestinal permeability was determined by measuring urinary CrEDTA excretion. Translocation of salmonella was quantified by analysis of urinary nitric oxide metabolites with time. Before infection, FOS increased mucosal lactobacilli and enterobacteria in cecum and colon, but not in the ileum. However, FOS increased cytotoxicity of fecal water and intestinal permeability. Moreover, FOS increased fecal mucin excretion and mucin concentrations in cecal and colonic contents, and in cecal mucosa before infection. After infection, mucin excretion and intestinal permeability in the FOS groups increased even further in contrast to the control group. In addition, FOS increased translocation of salmonella to extraintestinal sites. Thus, FOS impairs the intestinal barrier in rats, as indicated by higher intestinal permeability. Whether these results can be extrapolated to humans requires further investigation
. http://www.nutrition.org/cgi/content/abstract/135/4/837
Transolaktion durch Präbiotika
Präbiotika wie Fructooligosaccharide (FOS) erhöhen die Permeabilität des Darmes. Eine Untersuchung an Ratten ergab, dass Krankheitskeime wie Salmonellen leichter in den Organismus eindringen.
Ungeklärt bleibt , ob Präbiotika auf diesem Wege auch Allergien fördern können .
Auch wenn diese Frage bisher nicht geklärt ist, werden FOS munter empfohlen -> fatalerweise sind sie auch in Medikamenten oder Nahrungsergänzungsmitteln* zu finden, die bei Nahrungsmittelunverträglichkeiten und ihrer Magen-Darm-Symptomatik verordnet werden – sie werden sogar als besonders „förderlich“ beworben.
· z.B. BactoFlor – Oligofructose
· Lactobact
· Lactonova Darmflora Aktiv
· Lactovit
Uli
Dietary Fructooligosaccharides Increase Intestinal Permeability in Rats
Sandra J. M. Ten Bruggencate, Ingeborg M. J. Bovee-Oudenhoven1, Mischa L. G. Lettink-Wissink and Roelof Van der Meer
Nutrition and Health Program, Wageningen Center for Food Sciences/NIZO Food Research, 6710 BA Ede, The Netherlands
1To whom correspondence should be addressed. E-mail: Ingeborg.Bovee@NIZO.nl .
We showed previously that fructooligosaccharides (FOS) decrease the resistance to salmonella infection in rats. However, the mechanism responsible for this effect is unclear. Therefore, we examined whether dietary FOS affects intestinal permeability before and after infection with Salmonella enterica serovar Enteritidis. Male Wistar rats were fed restricted quantities of a purified diet that mimicked the composition of a Western human diet. The diet was supplemented with 60 g/kg cellulose (control) or 60 g/kg FOS and with 4 mmol/kg of the intestinal permeability marker chromium EDTA (CrEDTA) (n = 8 or 10). After an adaptation period of 2 wk, rats were orally infected with 108 colony-forming units (cfu) of S. enteritidis. Mucin concentrations in intestinal contents and mucosa were measured fluorimetrically, as markers of mucosal irritation. Intestinal permeability was determined by measuring urinary CrEDTA excretion. Translocation of salmonella was quantified by analysis of urinary nitric oxide metabolites with time. Before infection, FOS increased mucosal lactobacilli and enterobacteria in cecum and colon, but not in the ileum. However, FOS increased cytotoxicity of fecal water and intestinal permeability. Moreover, FOS increased fecal mucin excretion and mucin concentrations in cecal and colonic contents, and in cecal mucosa before infection. After infection, mucin excretion and intestinal permeability in the FOS groups increased even further in contrast to the control group. In addition, FOS increased translocation of salmonella to extraintestinal sites. Thus, FOS impairs the intestinal barrier in rats, as indicated by higher intestinal permeability. Whether these results can be extrapolated to humans requires further investigation
. http://www.nutrition.org/cgi/content/abstract/135/4/837
Transolaktion durch Präbiotika
Präbiotika wie Fructooligosaccharide (FOS) erhöhen die Permeabilität des Darmes. Eine Untersuchung an Ratten ergab, dass Krankheitskeime wie Salmonellen leichter in den Organismus eindringen.
Ungeklärt bleibt , ob Präbiotika auf diesem Wege auch Allergien fördern können .
Auch wenn diese Frage bisher nicht geklärt ist, werden FOS munter empfohlen -> fatalerweise sind sie auch in Medikamenten oder Nahrungsergänzungsmitteln* zu finden, die bei Nahrungsmittelunverträglichkeiten und ihrer Magen-Darm-Symptomatik verordnet werden – sie werden sogar als besonders „förderlich“ beworben.
· z.B. BactoFlor – Oligofructose
· Lactobact
· Lactonova Darmflora Aktiv
· Lactovit
Uli